0816010 Surbiopure Maxi Plasmid EF Kit
Endotoxin-Free Plasmid Large-Scale Preparation Kit (Magnetic Bead Method)
【Product Specification】2T/Box or 10T/Box
【Product Introduction】
This kit optimizes the traditional alkaline lysis method to obtain high-quality
plasmid DNA in 30 minutes. It uses nano magnetic beads that specifically bind
to DNA and a unique buffer system. The following procedures are suitable for
extracting plasmid DNA from 100-200 ml overnight cultures of Escherichia
coli. The yield and quality of plasmid extraction are related to factors suchas the
type and culture conditions of the host bacteria, cell lysis, plasmid copy number,
plasmid stability, and antibiotics.
Plasmid DNA extracted using this kit can be applied to various routine operations,
including restriction digestion, PCR, sequencing, ligation, transformation, library
screening, in vitro translation, and transfection of some conventional cell lines.
